“Click” functionalization DNA and dual genetic encoding of acetyl-lysine /thioacetyl-lysine mediated by Flexizyme

Dr. Hai Xiong

Organic and Bioorganic Chemistry, Osnabrück University

About the Speaker

Dr. Xiong got his PhD degree at the Osnabrück University, Germany. He is currently working as a Postdoctoral Associate at Yale University, USA. He has published papers as the first author at the Angew. Chem. Int. Ed., J. Am. Chem. Soc., Bioconjugate Chem., J. Org. Chem., Org. Biomol. Chem., Tetrahedron, et al.

Talk Introduction

8-Aza-7-deazapurine nucleosides with different terminal triple bonds in their side chains were synthesized and incorporated in oligonucleotides. Lately, fluorescence labeled DNA and cross-linked DNA(homodimer, heterdimer) were constructed by using copper(I)-catalyzed “bis-click” or “stepwise click” chemistry. The branched DNA(Y-DNA) units that formed dendritic nucleic acid structures were also prepared by a similar approach. Furthermore, it was also successful to create structurally well-defined DNA surface arrays (stable DNA nanopattern) by surface immobilization of acetylene-bearing oligodeoxynucleotides onto azido-terminated silicon wafer.

Lysine acetylation of histones and its crosstalk with other post-translational modifications(PTMs), are crucial to DNA replication, DNA repair, and transcriptional regulation. Acetyl-lysine(AcK) and the non-hydrolysable thioacetyl-lysine(ThioAcK) were site-specifically incorporated into human histone H3 at several amino acid positions both individually and simultaneously in vitro, mediated by Flexizyme. The method provides a powerful tool to study protein acetylation and its role in crosstalk between PTMs.

时间: 2016年3月29日下午 2:00-3:00

地点: 办公楼620-12

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