学院简介

深圳大学高等研究院是深圳大学于2014年成立的一个包含本科与研究生培养、侧重跨学科教学与学术研究的校内综合办学单位。作为深圳大学内部探索全面改革创新的学术特区,高等研究院与香港和海外著名高校合作,借鉴国内外研究型大学通行的管理模式,引进具有一流视野的资深教授和发展潜力的青年教师,营造与国际接轨的学术氛围和培养环境,开展卓越的教学、研究和管理工作。

新闻动态

高等研究院系列学术讲座之十三

发布时间:2015-01-04 | 浏览次数:

高等研究院学术讲座十三

 

Co-transcriptional recruitment of She2p by RNA pol II elongation factor Spt4-Spt5 promotes mRNA localization to yeast bud

Dr. Shen Zhifa, Ph.D.
University of Montreal

 

About the speaker

Dr. Shen received Ph.D. in Biochemistry at University of Montreal under the supervision of Dr. Pascal Chartrand. He then did his postdoctoral research with Dr. Shyam Sharan at NCI/NIH, USA. In 2011, Dr. Shen had his second Postdoctoral fellowship in McMaster University under the supervision of Dr.Yingfu Li. Currently he is a research associate in the Department of Biochemistry, University of Montreal.

 

Talk Introduction

Cytoplasmic transport and localization of messenger RNAs allows temporal and spatial expression of specific factors involved in cell fate determination, synaptic plasticity, cellular polarity or asymmetric cell division. In S. cerevisiae, the ASH1 mRNA is actively transported and localized to the bud tip of budding yeast. Proper ASH1 mRNA localization and Ash1p asymmetric expression involve localization factors and translational repressors. We used chromatin immunoprecipitation (ChIP) and found that She2p is recruited on the ASH1 gene during transcription, via its interaction with the nascent ASH1 mRNA. Puf6p is also recruited on the ASH1 gene, but in a She2p-dependent manner.  Interestingly, we detected an interaction between She2p and Rpb1p, the largest subunit of RNA polymerase II in vivo. This interaction is independent of the RNA-binding properties of She2p, and involves the elongating form of the RNA polymerase II. We also found that She2p interacts with both members of the elongation factors Spt4p /Spt5p. Altogether, our results show that factors involved in cytoplasmic mRNA localization are recruited co-transcriptionally on nascent mRNAs via interaction with the transcription machinery, pointing toward a role of the transcription machinery in the mRNA localization process.

 

 时间: 201519 下午14:00-15:00

地点办公楼207会议室

All are welcome