Co-transcriptional recruitment of She2p by RNA pol II elongation factor Spt4-Spt5 promotes mRNA localization to yeast bud
Dr. Shen Zhifa, Ph.D.
University of Montreal
About the speaker
Dr. Shen received Ph.D. in Biochemistry at University of Montreal under the supervision of Dr. Pascal Chartrand. He then did his postdoctoral research with Dr. Shyam Sharan at NCI/NIH, USA. In 2011, Dr. Shen had his second Postdoctoral fellowship in McMaster University under the supervision of Dr.Yingfu Li. Currently he is a research associate in the Department of Biochemistry, University of Montreal.
Cytoplasmic transport and localization of messenger RNAs allows temporal and spatial expression of specific factors involved in cell fate determination, synaptic plasticity, cellular polarity or asymmetric cell division. In S. cerevisiae, the ASH1 mRNA is actively transported and localized to the bud tip of budding yeast. Proper ASH1 mRNA localization and Ash1p asymmetric expression involve localization factors and translational repressors. We used chromatin immunoprecipitation (ChIP) and found that She2p is recruited on the ASH1 gene during transcription, via its interaction with the nascent ASH1 mRNA. Puf6p is also recruited on the ASH1 gene, but in a She2p-dependent manner. Interestingly, we detected an interaction between She2p and Rpb1p, the largest subunit of RNA polymerase II in vivo. This interaction is independent of the RNA-binding properties of She2p, and involves the elongating form of the RNA polymerase II. We also found that She2p interacts with both members of the elongation factors Spt4p /Spt5p. Altogether, our results show that factors involved in cytoplasmic mRNA localization are recruited co-transcriptionally on nascent mRNAs via interaction with the transcription machinery, pointing toward a role of the transcription machinery in the mRNA localization process.
时间: 2015年1月9日 下午14:00-15:00
All are welcome！