Hai Xiong*, Fengli Li, Linyu Zeng, and Qiyu Lei

Institute for Advanced Study, Shenzhen University, Shenzhen, 518060, P. R. China.

Email:hai.xiong@szu.edu.cn

Natural nucleic acids can encode information, possess desired properties, and provide many advanced structures and functions which contain aptamers owning the ability to bind specific targets, and DNAzymes/ribozymes catalyzing specific reactions. Further, DNA and RNA treated as biomarkers in buffer or blood plasma have been also demonstrated. In this review, we provided an overall summary of the emerging trends for functional COF nanomaterials as biosensors in the synthesis, properties, and applicationin DNA/RNA detection. More importantly, the construction of DNA/RNA hybridized COF nanoprobes and theirpotential applicationsin bioanalyte detection,biosensing, biomarker imaging (DNA, Aptamer, miRNA, siRNA, ATP, Human insulin, Exosomes, Nanozyme), and clinical biomedicine (antibiotics, microenvironment-responsive drug release) were also summarized. In brief, this review describes the current state of research, highlights recent advancements, discusses challenges and limitations, and suggests future research directions for the COFs nanoprobes conjugating DNA/RNA.

Graphic Abstract: The synthesis, properties of COFs and their applications in DNA/RNA detection are described with various sensing techniques in this review. The DNA/RNA hybridized COF nanoprobes have potential applications in the bioanalyte detection, clinical biomedicine, and biomarker imaging are also summarized.

Prof. Hai XiongatInstitute for Advanced Study in Shenzhen University is the first and corresponding author.This work is supported by the Science and Technology Innovation Commission of Shenzhen, China (JCYJ20210324095607021), and the Special Project of Key Fields of Universities in Guangdong Province, China (2021ZDZX2047).

The research paper linked inhttps://doi.org/10.1016/j.trac.2023.117414