报告主题：整合空间基因组学与靶向测序突破微生物组瓶颈的探索研究

主 讲 人：夏雨 副教授（南方科技大学）

主 持 人：张新旭

时 间：2025年9月29日周一10：00

地 点：致知楼207

嘉宾简介：

夏雨，香港大学博士。现任南方科技大学，环境科学与工程学院，长聘副教授，博士生导师，环境微生物与生态基因组学实验室负责人（XYLab）。近年来在Environmental Science & Technology, Water Research, Genome Research等期刊发表一作/通讯论文50余篇，总引用次数 4900余次(Google Scholar，h-index 31)。现任iMeta期刊及Frontiers in Environmental Science期刊副主编，中国工程院院刊Engineering及mLife期刊青年编委。主持国自然面上项目、青年项目各一项，并担任两项国家重点研发计划课题负责人。获广东省“青年拔尖人才”，深圳市“海外高层次人才”，南方科技大学“研究生优秀导师”,“优秀书院导师”等荣誉。曾担任美国微生物协会香港地区青年大使。

报告摘要：

This talk will cover two aspects of XYlab’s research. The first focus is on elucidating the microbial interactions within complex microbiomes via micron-scale cluster sampling (MSC)-based spatial omics. It is generally believed that interspecies interactions are occurring within micron-scale cell aggregates, approximately 100 µm in size. Unlike previous studies that have largely concentrated on bulk-scale observations (~cm), we have constructed ecological interaction networks of microbiomes at a much finer resolution of 40-70 µm. This approach enables us to reliably detect genuine interactions between different taxa within various engineering communities, such as anammox and anerobic digestion microbiomes. Moreover, by employing MSC, we started to gain insights into the often-overlooked microbial spatial heterogeneity, which plays a crucial role in community assembly and succession. The second part of the talk addresses the challenge of detecting rare species in metagenomic investigations. Despite their ecological importance, rare species' genomes remain difficult to retrieve due to their low abundance. Our solution, metaRUpore, leverages Oxford Nanopore Technologies’ (ONT) adaptive sampling capability to dynamically reduce sequencing coverage of known host and dominant microbial populations during sequencing. This targeted approach significantly improves the recovery of near-finished metagenome-assembled genomes (MAGs) from rare taxa, positioning metaRUpore as a promising standard for future metagenomic sequencing.