Education

1988 Ph. D. in Microbiology. University of Kent at Canterbury, Kent, U.K.

1984 MPhil. in Biology. The Chinese University of Hong Kong, Shatin, N.T., Hong Kong.

1983 UNESCO Postgraduate diploma in Microbiology and Biotechnology. University of Tokyo/Osaka University, Japan.

1980 B.Sc. (Honors in Genetics). University of Alberta, Edmonton, Canada.

Working Experience

8/2016-4/2019 Lecturer (Fractional appointment) in the School of Biological Sciences, The University of Hong Kong (HKU)

7/2016-8/2017 Honorary Associate Professor in the School of Biological Sciences, HKU

7/2007-6/2016 Associate Professor in the School of Biological Sciences, HKU

12/1999-6/2007 Associate Professor in the Department of Botany, HKU

8/1994-11/1999 Lecturer/Assistant Professor in the Department of Botany, HKU

11/1993 -7/1994 Group Leader in Biomedical Research Centre, University of Dundee, United Kingdom

10/1987-10/1993 Postdoctoral research associate in the Department of Biochemistry, University of Oxford, United Kingdom

10/1986-12/1986 Part time demonstrator in Biological Laboratory, University of Kent at Canterbury.

9/1981-9/1982 Part time demonstrator in the Biology Department, The Chinese University of Hong Kong (CUHK)

9/1983-8/1984 Part time demonstrator in the Biology Department, CUHK

9/1980-8/1981 Full time demonstrator in the Biology Department, CUHK

Scientific Affiliation

Life Member of the Microbiology Society, UK.

Life Member of the Genetics Society, UK.

Merit

1984-1987 Recipient of an Overseas Research Scheme Award funded by the Committee of Vice-Chancellors and Principals of the Universities of the United Kingdom Recipient of a University of Kent Studentship.

1982-1983 Recipient of a postgraduate research fellowship funded by UNESCO, the ICRO and the Japanese Government.

1992 A travel/subsistence grant was provided by the Royal Society to attend the 16th International Conference on Yeast Genetics and Molecular Biology in Vienna.

1992 A bursary was provided by the Marine Biological Association of the United Kingdom (MBA) to study helix-loop-helix proteins in marine diatoms.

2006 Recipient for an Award for Teaching Excellence from the Faculty of Science, HKU.

Publications

1.Tsang, J.S.H., Sallis, P.J., Bull, A.T. and Hardman, D.J. 1988. A mono bromoacetate dehalogenase from Pseudomonas cepacia MBA4. Arch. Microbiol. 150, 441 446.

2.Chambers, A.,Tsang, J.S.H., Stanway, C., Kingsman, A.J. and Kingsman, S.M. 1989. Transcriptional control of Saccharomyces cerevisiae PGK gene by RAP1. Molec. Cell. Biol. 9, 5516 5524.

3.Henry, Y.A.L., Chambers, A.,Tsang, J.S.H., Kingsman, A.J. and Kingsman, S.M. 1990. Characterisation of the DNA binding domain of the yeast RAP1 protein. Nucleic Acids Res. 18, 2617 2623.

4.Chambers, A., Stanway, C.,Tsang, J.S.H., Henry, Y.A.L, Kingsman, A.J. and Kingsman, S.M. 1990. ARS binding factor 1 binds adjacent to RAP1 at the UASs of the yeast glycolytic genes PGK and PYK1. Nucleic Acids Res. 18, 5393 5399.

5.Tsang, J.S.H., Henry, Y.A.L., Chambers, A., Kingsman, A.J. and Kingsman, S.M. 1990. Phosphorylation influences the binding of the yeast RAP1 protein to the UAS of the PGK gene. Nucleic Acid Res. 18, 7331 7337.

6.Murdiyatmo, U., Asmara, W.,Tsang, J.S.H., Baines, A.J., Bull, A.T. and Hardman, D.J. 1992. Molecular biology of the 2 haloacid halidohydrolase IVa from Pseudomonas cepacia MBA4. Biochem. J. 284, 87 93.

7.Dowell, S.J.,Tsang, J.S.H. and Mellor, J. 1992. The centromere and promoter factor 1 of yeast contains a dimerisation domain located carboxy terminal to the bHLH domain. Nucleic Acids Res. 20, 4229 4236.

8.Kent, N.A.,Tsang, J.S.H., Crowther, D.J. and Mellor, J. 1994. Chromatin structure modulation in Saccharomyces cerevisiae by centromere and promoter factor 1. Molec. Cell. Biol. 14, 5229 5241.

9.Kong, K.F. andTsang, J.S.H. 1998. Nucleotide sequences of cDNAs (AJ010160, AJ010161 and AJ010162) encoding a Type 2 metallothionein-like protein from water hyacinth Eichhornia crassipes. Plant Physiol 118: 1101.

10.Kong, K.F. andTsang, J.S.H. 1999. Nucleotide sequences of genomic clones (Accession nos. AJ247090, AJ247195 and AJ247196) encoding a type 2 metallothionein-like protein from water hyacinth Eichhornia crassipes. Plant Physiol 120:1207.

11.Tsang, J.S.H. and Sam, L. 1999. Cloning and characterization of a cryptic haloacid dehalogenase gene from Burkholderia cepacia MBA4. J. Bacteriol. 181:6003-6009.

12.Tsang, J.S.H. and Pang, B.C.M. 2000. Identification of the dimerization domain of dehalogenase IVa of Burkholderia cepacia MBA4. Appl. Environ. Microbiol. 66:3180-3186.

13.Pang, B.C.M andTsang J.S.H. 2001. Mutagenic analysis of the conserved residues in dehalogenase IVa of Burkholderia cepacia MBA4. FEMS Microbiol. Lett. 204:135-140.

14.Tsang, J.S.H. and Sze, J. 2002. Sec-dependent and Sec-independent translocation of haloacid dehalogenase Chd1 of Burkholderia cepacia MBA4 in Escherichia coli. FEMS Microbiol. Lett. 211:259-264.

15.Tsang, J.S.H. 2004. Molecular biology of the Burkholderia cepacia complex. Adv. Appl. Microbiol. 54: 71-91.

16.Schmidberger, J.W., Oakley, A.J.,Tsang, J.S.H. and Wilce, M.C.J. 2005. Purification, crystallization and preliminary crystallographic analysis of DehIVa, a dehalogenase from Burkholderia cepacia MBA4. Acta Cryst. F 61:271-273.

17.Chan, Y.H., Kwok, A.C.M., Tsang,J.S.H. and Wong, J.T.Y. 2006. Alveolata histone-like proteins have different evolutionary origins. J. Evol. Biol. 19:1717-1721.

18.Yu, M. andTsang, J.S.H. 2006. Use of ribosomal promoters from Burkholderia cenocepacia and Burkholderia cepacia for improved expression of transporter protein in Escherichia coli. Protein Exp. Purif. 49:219-227.

19.Kwok, C.S.Y., Siu, A.F-M., Ngai,S.M., Che, C-M. andTsang, J.S.H. 2007. Proteomic analysis of Burkholderia cepacia MBA4 in the degradation of monochloroacetate. Proteomics 7:1107-1116.

20.Schmidberger J.W., Wilce J.A.,Tsang J.S.H. and Wilce M.C.J. 2007. Crystal structures of the substrate free-enzyme, and reaction intermediate of the HAD superfamily member, haloacid dehalogenase DehIVa from Burkholderia cepacia MBA4. J. Mol. Biol. 368:706-717.

21.Faan, Y-W., Yu, M. andTsang, J.S.H. 2007. Blue-white selection of regulatory genes that affect the expression of dehalogenase IVa of Burkholderia cepacia MBA4. Appl. Microbiol. Biotech. 76:429-437.

22.Yu, M., Faan, Y-W., Chung, W.Y.K. andTsang, J.S.H. 2007. Isolation and characterization of a novel haloacid permease from Burkholderia cepacia MBA4. Appl. Environ. Microbiol. 73:4874-4880.

23.Ho, P., Kong, K.F., Chan, Y.H.,Tsang, J.S.H. and Wong, J.T.Y. 2007. An unusual S-adenosylmethionine synthetase gene from dinoflagellates is methylated. BMC Molecular Biology 8:87.

24.Yeung, S-L., Cheng, C., Lui, T.K.O.,Tsang, J.S.H., Chan, W-T. and Lim, B.L. 2009. Purple acid phosphatase-like sequences in prokaryotic genomes and the characterization of an atypical purple alkaline phosphatase from Burkholderia cenocepacia J2315. Gene 440:1-8.

25.Tse, Y.M., Yu, M andTsang, J.S.H. 2009. Topological analysis of a haloacid permease of a Burkholderia sp. bacterium with a PhoA-LacZ reporter. BMC Microbiology 9:233.

26.Su, X., Kong, K-F. andTsang J.S.H. 2012. Transports of acetate and haloacetate in Burkholderia species MBA4 are operated by distinct systems. BMC Microbiology 12:267.

27.Su, X. andTsang, J.S.H. 2013. Existence of a robust haloacid transport system in a Burkholderia species bacterium. BBA-Biomembranes 1828(2):187–192.

28.Su, X., Deng, L., Kong, K.F. andTsang J.S.H. 2013. Enhanced degradation of haloacid by heterologous expression in related Burkholderia species. Biotechnol. Bioeng. 110(10): 2687-2696.

29.Pan, Y., Kong, K.F. andTsang, J.S.H. 2014. Draft genome sequence of the haloacid-degrading Burkholderia caribensis strain MBA4. Genome Announcements 2(1):e00047-14.

30.Pan, Y., Kong, K.F. andTsang, J.S.H. 2015. Complete genome sequence and characterization of the haloacid-degrading Burkholderia caribensis MBA4. Standards in Genomic Sciences 10:114.

31.Pan, Y., Kong, K.F. andTsang, J.S.H. 2016. Complete genome sequence of the exopolysaccharide-producing Burkholderia caribensis type strain MWAP64. Genome Announcements 4(1):e01636-15. doi:10.1128/genomeA.01636-15.

32.Su, X., Li, R. andTsang J.S.H. 2016. The 228 bp upstream non-coding region of haloacids transporter gene dehp2 has regulated promoter activity. Gene 593:322-329.

33.Su, X., Li, R. andTsang J.S.H. 2016. Transport of haloacids across biological membranes. BBA-Biomembranes 1858:3061-3070.