报告主题：发展单细胞测序技术并解析肿瘤微环境

主讲人：靳文菲研究员（中国科学院上海营养与健康研究所）

主持人：李文金

时 间：2024年6月25日周二14：30

地 点：深圳大学致知楼706

嘉宾简介：

中国科学院上海营养与健康研究所研究员,国家高层次青年人才。2012年获中国科学院-马普学会计算生物学伙伴研究所博士学位。2013-2017年在美国国立卫生研究院(NIH)从事博士后研究，2017年受聘南方科技大学研究员。2024年加入中国科学院上海营养与健康研究所。靳博士长期从事基因组、生物信息、肿瘤免疫相关研究。注重开发和整合单细胞测序技术和计算方法，并使用单细胞技术解析细胞异质性和肿瘤微环境等。发表论文50余篇，其中以通讯或第一作者身份在Nature、Nature Cancer、Nature Methods、Mol Cell、PNAS、Dev Cell、Genome Res、Genome Biol等杂志发表论文30余篇。先后承担十余项国家和省部级项目（包括主持4项国自然和参与3项国家重点研发计划）。

靳博士曾获南方科技大学首届“良师益友”优秀研究生导师、NHLBI' Orloff创新奖、NIH杰出科研奖、Springer Theses、中科院百篇优博、ICHG/ASHG Travel Award、南科大优秀研究生导师、优秀书院导师等十几种荣誉。

报告摘要：

Single cell sequencing provided exciting insights into biology and medicine at the unitary resolution of life. We developed a series of singe cell technologies including 1) single cell DNase sequencing (scDNase-Seq); 2) single cell polyadenylation sequencing (scPolyA-seq); 3) in situ SHERRY after ATAC-seq (ISSAAC-seq); 4) counterpart composite index (CCI) for mapping cancer cells onto reference lineages. By performing single cell RNA-seq of tumor-infiltrating immune cells from colorectal cancer patients, we found that tumor-associated macrophages (TAMs) increased most compared to their counterparts in normal tissue and displayed the highest immune-inhibitory signatures among all immunocytes. Intriguingly, Sirpa-/- mice were more resistant to solid tumor progression than wild-type mice. Moreover, Sirpa deficiency reprogramed the tumor microenvironment. Sirpa-/- macrophages presented strong antitumor activity and antigen presentation to enhance T cell activation and proliferation. Therefore, these findings suggest that Sirpa deletion enhances innate and adaptive immune activation through a CD47-independent mechanism, and Sirpa blockade could be a promising strategy to improve cancer immunotherapy efficacy.